Analysis of DNA extraction protocols in tissues for studying physical properties

• Grant number: 07/01622-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2007
• End date: December 31, 2008
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Herculano da Silva Martinho
• Grantee: Bianca Fogazza Palma
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

The Raman spectroscopy technique jointly with magnetization, specific heat and resistivity experiments could be very useful to study the physical properties of biomolecules as aminoacids, proteins, and DNA. From these properties could be possible to understand the mechanism of several pathologies, in special the cancerous process. In this project we propose the systematic analysis of three human tissues DNA extraction protocols (DNA commercial kits of Qiagen and Invitrogen and in house method of Phenol-Chloroform) with objective of: (I) evaluate the influence of the residual protocols reagents on the physical properties, specially the Raman spectra; (II) quantify the performance of the extraction; and (III) optimize and/or implement new extraction protocol fitting the proposed needs.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PALMA‚ B.F.; FERRARI‚ A.B.; BITAR‚ R.A.; CARDOSO‚ M.A.; MARTIN‚ A.A.; DA SILVA MARTINHO‚ H.. DNA extraction systematics for spectroscopic studies. SENSORS, v. 8, n. 6, p. 3624-3632, 2008. (07/01622-4)