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Synthesis, characterization and biological properties of a photosensitizing ruthenium mononuclear complex with a bulky ligand and a DNA intercalator with photoinduced release of NO

Grant number: 20/15607-1
Support type:Scholarships in Brazil - Master
Effective date (Start): June 01, 2021
Effective date (End): May 31, 2023
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
Principal researcher:Sofia Nikolaou
Grantee:Renan Ribeiro Bertoloni
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This project proposes the synthesis, characterization and investigation of biological properties of interest for an unprecedented complex of molecular formula [Ru (terpy) (dppz) NO] (PF6) 3 (terpy = 2,2 ': 6'2 "-terpiridine and dppz = dipyride [3,2-a: 22,32-c] phenazine). The choice of the metallic center and each ligand was made based on their individual properties, aiming at a synergy that makes the compound an excellent candidate for a metallodrug. The photoinduced release of NO is expected to have a high quantum yield, since the biological properties of this molecule are of extreme interest, and the introduction of the dppz, planar and hydrophobic ligand, intends to make the compound interact with the DNA and / or HSA with a high binding constant, which can lead to anti-cancer activities of interest. Finally, ruthenium compounds are great candidates for photosensitizers, so the quantum yield measurement of the compound's singlet oxygen generation is also a property of interest to be studied. Since the title compound is unprecedented, the synthetic and purification route will be developed within this project. The characterization will be carried out using the techniques of spectroscopy in the region of UV-Vis, IR, cyclic and differential pulse voltammetry, NMR, elemental analysis and mass spectrometry. The biological properties will be investigated through spectrophotometric monitoring of NO photolysis and the generation of singlet oxygen, titration of the complex with DNA and suppression of fluorescence with HSA. Characterization data, singlet oxygen production capacity and interaction with biomolecules of the [Ru (terpy) (dppz) Cl] PF6 complex will be used for comparison. (AU)

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