- Research Grants
|Support type:||Scholarships in Brazil - Post-Doctorate|
|Effective date (Start):||September 01, 2010|
|Effective date (End):||August 31, 2013|
|Field of knowledge:||Biological Sciences - Biochemistry|
|Principal Investigator:||Andrea Balan Fernandes|
|Home Institution:||Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia, Inovações e Comunicações (Brasil). Campinas , SP, Brazil|
In recent studies carried through by our group involving proteins of the ABC transport systems from Xanthomonas axonopodis pv. citri (X. axonopodis), we identified by amino acid sequence homology, a set of proteins that supposedly would form the "cys regulon" for sulphur assimilation, well characterized in Escherichia coli. Despite of the economic importance of X. axonopodis, a phytopathogenic bacterium responsible for the development of the citrus canker disease in plants, much little is known about the metabolism of this bacterium in relation to the captation of ions and in special, the sulphur metabolism. The proteins identified in this regulon, constitute three ABC transporters: sulphate transport (SbpCysWAR), alkanesulfonates (SsuABC) and aliphatic sulfonates (TauABC), as well as all the necessary enzymes for the conversion of that molecules in sulfites and alkane, and their transcription regulators. This work is presented in two parts. In the first one, it is intended to characterize all the ways of capture and assimilation of sulphur using molecular modeling and "docking" for studies of interaction with ligands and protein-protein. And in the second part, real-time PCR and mass spectroscopy experiments will be carried out for identification of the expressed proteins in different growth conditions.