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Structural and funcional characterization of the sulfate ABC transporter from Xanthomonas axonopodis pv. citri and related enzymes

Grant number: 13/09172-9
Support Opportunities:Regular Research Grants
Duration: August 01, 2013 - July 31, 2015
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Andrea Balan Fernandes
Grantee:Andrea Balan Fernandes
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


This project aims to characterize functionally and structurally the pathway of sulfate assimilation in Xanthomonas citri, the causal agent of citrus canker, with emphasis on the ABC transporter involved in the uptake of this ion and enzymes responsible for the oxide/ reduction to sulfide. The research is based on two main steps aimed at, first, for the characterization of proteins involved in the pathway of assimilation (ABC transporter) and oxidereduction of the inorganic sulphate (enzymes Cys), to elucidate their structural and functional characteristics highlighting the interactions with proteins and ligands involved in the transport and / or ion metabolism. Secondly, we study specifically the activation of this pathway in different culture conditions in vitro and in vivo during infection in the plant. The activation of sbpcysWUA operon, which encodes the sulfate ABC transporter in X. citri, and expression of the proteins CysD, CysNC, CysG, CysI, and CysH CYSJ will be studied by expression of green fluorescent protein gene (eGFP) controlled by the promoter region of the operon by means of RT-PCR techniques and proteomics. Expect that the results obtained in this study represent an important contribution on the physiology and genetics of X. citri and increase knowledge about the role of sulfate in behavior in vitro and in vivo of this important pathogen. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, CRISTIANE TAMBASCIA; ROESLER, CASSIA; FARIA, JESSICA NASCIMENTO; FESSEL, MELISSA REGINA; BALAN, ANDREA. Sulfate-Binding Protein (Sbp) from Xanthomonas citri: Structure and Functional Insights. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 30, n. 7, p. 578-588, . (13/09172-9, 13/16094-4)
SAMPAIO, ALINE; PEGOS, VANESSA RODRIGUES; OSHIRO, ELISA EMIKO; BALAN, ANDREA. The periplasmic binding protein NrtT affects xantham gum production and pathogenesis in Xanthomonas citri. FEBS OPEN BIO, v. 7, n. 10, p. 1499-1514, . (13/09172-9, 11/22386-2, 11/20468-1)
PEGOS, VANESSA R.; SANTOS, RODRIGO M. L.; MEDRANO, FRANCISCO J.; BALAN, ANDREA. Structural features of PhoX, one of the phosphate-binding proteins from Pho regulon of Xanthomonas citri. PLoS One, v. 12, n. 5, . (13/09172-9, 11/20468-1)
PEREIRA, CRISTIANE TAMBASCIA; MOUTRAN, ALEXANDRE; FESSEL, MELISSA; BALAN, ANDREA. The sulfur/sulfonates transport systems in Xanthomonas citri pv. citri. BMC Genomics, v. 16, . (13/09172-9, 10/09812-0, 10/14207-8)

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