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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The periplasmic binding protein NrtT affects xantham gum production and pathogenesis in Xanthomonas citri

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Author(s):
Sampaio, Aline [1, 2] ; Pegos, Vanessa Rodrigues [2, 3] ; Oshiro, Elisa Emiko [4] ; Balan, Andrea [4]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Programa Interunidades Biotecnol, Sao Paulo - Brazil
[2] Ctr Pesquisas Energia & Mat CNPEM, Lab Nacl Biociencias LNBio, Sao Paulo - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Postgrad Program Genet & Mol Biol, Campinas, SP - Brazil
[4] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Av Prof Lineu Prestes 1374, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FEBS OPEN BIO; v. 7, n. 10, p. 1499-1514, OCT 2017.
Web of Science Citations: 0
Abstract

In Xanthomonas citri, the bacterium that causes citrus canker, three ATP-binding cassette (ABC) transporters are known to be dedicated to the uptake of sulfur compounds. In this work, using functional, biophysical and structural methods, we showed that NrtT, a periplasmic component of the ABC transporter NrtCB, is an alkanesulfonate-binding protein and that the deletion of the nrtT gene affected xantham gum synthesis, adhesion and biofilm production, similarly to the phenotype obtained in the X. citri ssuA-knockout strain, in which the alkanesulfonate-binding protein SsuA is absent. Although NrtA and SsuA share similar ligands, the function of these proteins is not complementary. These results emphasize that organic-sulfur sources are directly involved with bacterial infection in vivo and are needed for pathogenesis in X. citri. (AU)

FAPESP's process: 13/09172-9 - Structural and funcional characterization of the sulfate ABC transporter from Xanthomonas axonopodis pv. citri and related enzymes
Grantee:Andrea Balan Fernandes
Support type: Regular Research Grants
FAPESP's process: 11/22386-2 - Structural caracterization and functional analysis of nitrate/nitrite transporter ABC type of Xanthomonas axonopodis PV. citri
Grantee:Aline Sampaio Pinto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/20468-1 - Molecular mechanisms involved in pathogen adaptation and virulence, host resistance and symptom development in citrus-bacteria interactions
Grantee:Celso Eduardo Benedetti
Support type: Research Projects - Thematic Grants