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Structural and functional studies of periplasmic binding proteins and their interaction with the membrane components of ABC transport systems from Xanthomonas axonopodis PV. citri and Xanthomonas campestris PV. campestris

Grant number: 10/04883-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): May 01, 2010
Effective date (End): June 30, 2011
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Andrea Balan Fernandes
Grantee:Carolina Santacruz Perez
Home Institution: Laboratório Nacional de Luz Síncrotron (LNLS). Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia, Inovações e Comunicações (Brasil). Campinas , SP, Brazil

Abstract

The study of the phytopathogenic bacteria genomes of Xanthomonas axonopodis pv. citri (Xac) and Xanthomonas campestris pv. campestris (Xcc) disclosed that 4% of the same codify proteins of the ABC family transporters and that both bacteria, although phylogenetically related, show significant differences in the transporters content. The characterization of these systems is of great importance for the understanding of many functional and physiological aspects of organisms, as well as for the development of transport inhibitors that affects the bacterial growth, and drugs of interest. In recent years, with the expansion of Structural Biology and of projects directed toward the development of new drugs, these transporters have been an important research target. Focusing on the Protein Structural Biology field, our group has been working on the structural and functional characterization of the Xac periplasmic components, responsible for the transport systems affinity and specificity, as well as with some membrane components using Molecular Modeling tools. In this project, it is intended to continue this work including diverse periplasmic proteins of transporters that are present in Xac and Xcc. In Xac, much little is known about the interaction mechanism between the periplasmic protein and the membrane component. This work searches to consider/identify the possible interaction regions between both components, through the resolution of the periplasmic proteins crystallographic structures and the molecular modeling of the respective permeases. The modeling and functional/structural classification of ABC transport systems from Xac and Xcc will be interesting for studies on function, transport, specificity, pathogeneses and infectivity of the bacteria in its host.