Evalution of C. albicans cariogenicity isolated from pedriatric patients with sickle cell disease.

• Grant number: 09/14097-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2009
• End date: October 31, 2011
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Fernanda Lourenção Brighenti
• Grantee: Amanda Coelho Medeiros
• Host Institution: Faculdade de Odontologia (FOSJC). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Due to the increased risk for infections, patients with sickle cell disease need to take the antibiotic for a long period of time. The aim of this study is to evaluate de acidogenicity and extracellular polysaccharides production of C. albicans biofilms previously isolated from 25 children with sickle cell disease aged between 4 and 6 years old. For comparisons, 25 samples from healthy patients from the same gender and age ± 2 years will be included. The isolates will be stored at -20°C in BHI broth with glycerol 10% until analysis. Species identified as C. albicans will be analyzed regarding lactic acid and extracellular polysaccharides production in biofilm. The biofilms will grow for five days in microtiter plates. The medium will be refreshed on a daily basis. The lactic acid production will be analyzed by the enzymatic method. The extracellular polysaccharides present on dried biofilm and will be extracted with NaOH 1.0 mol L-1. The carbohydrates concentration will be colorimetrically determined. The data will be analysed with the software GraphPad Prism 3.02. The significance level will be set at 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRIGHENTI, FERNANDA LOURENCAO; MEDEIROS, AMANDA COELHO; MATOS, BRUNO MELLO; ABREU RIBEIRO, ZULENE EVELINE; KOGA-ITO, CRISTIANE YUMI. Evaluation of caries-associated virulence of biofilms from Candida albicans isolated from saliva of pediatric patients with sickle-cell anemia. Journal of Applied Oral Science, v. 22, n. 6, p. 484-489, NOV-DEC 2014. (09/14097-0)