Effects of flavonoids derived from Baccharis retusa treatment in a model of ovalbumin-induce chronic allergic pulmonary inflammation induced in mice

• Grant number: 10/14831-3
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): November 01, 2010
• Effective date (End): January 31, 2014
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Carla Máximo Prado
• Grantee: Camila Pivari Pedroso Sakoda
• Home Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Asthma is a disease with high prevalence, morbidity and causes high costs with hospitalization and treatment for governments. Although the treatment recommended by WHO is the corticosteroid, a potent anti-inflammatory, it has side effects and do not accrue all histopathological changes observed in asthma, such as lung remodeling. This study intend to demonstrate whether treatment with flavonoid isolated from the plant Baccharis retusa (5,6,7-trihydroxy-4-methoxy-flavanone) modulates the chronic allergic pulmonary inflammation in BALBc mice. Animals will be divided into groups: Group OVA or saline (control). These will be submitted to two intraperitoneal injections of saline or antigen diluted in aluminum hydroxide adjuvant (days 0 and 14) and four inhalations of 1% OVA or saline (days 22, 24, 26 and 28); From the first inhalation with ovalbumin, animals will be divided into Group OVA / flavonoid that will receive intranasal flavonoid treatment for 8 consecutive days), Group OVA / Vehicle, that will be treated with DMSO at the same concentration (vehicle) or Group OVA / dexamethasone that will receive subcutaneous injections of dexamethasone for 7 days consecutive. 24h after the last inhalation, mice will be anesthetized, and lung mechanics, bronchoalveolar lavage, and histological analysis will be performed. We expected that treatment with flavonoid, a potent antioxidant, reduces the changes induced by ovalbumin

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDROSO SAKODA, CAMILA PIVARI; DE TOLEDO, ALESSANDRA CHOQUETA; PERINI, ADENIR; PINHEIRO, NATHALIA MONTOURO; HIYANE, MEIRE IOSHIE; GRECCO, SIMONE DOS SANTOS; LOPES CALVO TIBERIO, IOLANDA DE FATIMA; SARAIVA CAMARA, NIELS OLSEN; MARTINS, MILTON DE ARRUDA; GHILARDI LAGO, JOAO HENRIQUE; RIGHETTI, RENATO FRAGA; PRADO, CARLA MAXIMO. Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation. ACTA HISTOCHEMICA, v. 118, n. 6, p. 615-624, 2016. Web of Science Citations: 6.

SANTANA, FERNANDA PAULA R.; PINHEIRO, NATHALIA M.; MERNAK, MARCIA ISABEL B.; RIGHETTI, RENATO F.; MARTINS, MILTON A.; LAGO, JOAO H. G.; DOS SANTOS LOPES, FERNANDA D. T. Q.; TIBERIO, IOLANDA F. L. C.; PRADO, CARLA M. Evidences of Herbal Medicine-Derived Natural Products Effects in Inflammatory Lung Diseases. Mediators of Inflammation, 2016. Web of Science Citations: 7.