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| Author(s): |
Adriana Moreira Soares
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2014-06-06 |
| Examining board members: |
Victor Hugo Aquino Quintana;
Eurico de Arruda Neto;
Adriano Mondini
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| Advisor: | Victor Hugo Aquino Quintana |
| Abstract | |
Dengue is an infectious disease caused by dengue virus (DENV) and transmitted mainly by Aedes aegypti mosquitoes. Dengue is the viral disease transmitted by arthropods of greater importance in public health, particularly affecting the tropical and subtropical countries of the world. Dengue epidemics have increased considerably in recent years throughout the world and the borders of virus circulation have been expanding constantly. Thus studies of different aspects of the disease and the virus are of great importance to improve knowledge about this threat. In this sense, it is important that some analyzes such as phylogeny and evolution be carried out to identify the circulating genotypes, their origin, their relationship with other subtypes and evolution along the time. This study aimed to analyze the phylogenetic and evolutionary relationships of DENV isolated in Ribeirão Preto between 2010 and 2011. Serum samples (n = 79) of dengue patients stored at -80°C were inoculated into C6/36 cells for virus isolation attempts, which was confirmed from 39 samples by indirect immunofluorescence and/or real-time RT-PCR. Sequencing of part of the viral NS5 gene protein or the E gene protein showed that 25 belonged to DENV-1, six to DENV-2 and eight to DENV-3. For the phylogenetic and evolutionary analyzes, the E gene protein of 15 DENV-1, four DENV-2 and one DENV-3 was sequenced. These analyzes were also carried out with the entire coding region of two DENV-1, one DENV-2 and one DENV-3. The analysis showed that all viruses were introduced in Ribeirão Preto from viruses originated in the state of Rio de Janeiro. Two lineages of DENV-1, one of DENV-2 and one of DENV-3 circulated between 2010 and 2011. The phylogenetic relationship of these viruses was similar regardless of the use of the E gene protein or the entire coding region sequences. (AU) | |
| FAPESP's process: | 11/04299-5 - Molecular characterization of dengue virus isolated in Ribeirão Preto from 2009 to 2012 |
| Grantee: | Adriana Moreira Soares |
| Support Opportunities: | Scholarships in Brazil - Master |