Analysis of JUN and FOS gene expression in adult and pediatric adrenocortical tumor cells.

Author(s)

Marlene Aparecida Ferreira Pinto

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-08-21

Examining board members

Claudimara Ferini Pacicco Lotfi; Camila Squarzoni Dale; Maria Lucia Cardillo Correa Giannella

• Advisor: Claudimara Ferini Pacicco Lotfi

Abstract

In order to have a better comprehension of adrenocortical tumor biology, molecular markers are utillized because they are in general cell cycle regulators. AP-1 is a dimeric factor, compound mainly by JUN (JUN, JUNB, JUND) and FOS proteins. In the present study, our hypothesis is that JUN proteins are correlated with others cell cycle regulatory proteins and could be utilized as a prognose and diagnostic predictor for adrenocortical tumors. To confirm our hypothesi the aim was genic and protein profile analyzes (PCR and Immunobloting). The PCR Array analysis showed that JUN and FOS gene expression were down regulated in these adrenocortical tumors cells which were confirmed through qPCR analysis. The genes expression analysis wasn´t able to stablish a standard of expression between the studied cell cultures or even between adults and pediatric tumors. The ACTH treatments increased JUN and JUNB proteins that may have some significance in responsive tumors to this hormone, and deserve further analysis. (AU)

• FAPESP's process: 11/15777-5 - Study of gene and protein expression of the transcription factor AP-1 in cell culture of adrenocortical tumors
• Grantee: Marlene Aparecida Ferreira Pinto
• Support Opportunities: Scholarships in Brazil - Master