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| Author(s): |
Karina Thieme
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-07-15 |
| Examining board members: |
Maria Oliveira de Souza;
Niels Olsen Saraiva Câmara;
Ana Paula Couto Davel;
Deise Carla Almeida Leite Dellova;
Maria Tereza Nunes
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| Advisor: | Maria Oliveira de Souza |
| Abstract | |
The aim of this study was to evaluate the morphofunctional effects of leptin treatment for 7 and 28 days on kidneys and the participation of the Renin-Angiotensin System (RAS) in this process. The animals treated for 7 days showed a reduction in weight gain (p<0.05), enhancement of blood pressure (BP; p<0.05), without changes in renal plasmatic flow (RPF) and glomerular filtrtaion rate (GFR). They also showed an increase in filtration fraction (FF) (p<0.05), diuresis and natriuresis. In the treatment for 28 days, there was an increase in BP (p<0.05), decrease in RPF (p<0.05), no changes in GFR and enhacement in FF (p<0.05). Both groups showed glomerular hypertrophy, increased desmin staining and proteinuria. The in vitro studies showed that leptin (100 and 250 ng/ml for 72 hours) induced mesangial cells apoptosis. In conclusion, the treatment with leptin for 7 and 28 days induced renal morphofunctional changes and they are mostly normalized by Losartan, demonstrating an interaction between leptin and systemic RAS, in this experimental model. (AU) | |
| FAPESP's process: | 10/51117-7 - O tratamento com leptina por 7 e 28 dias altera a função e a morfologia renal de ratos |
| Grantee: | Karina Thieme |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |