Study of amidasic activities present in Photorhabdus luminescens luminescens strain MN7, isolated from Heterorhabditis baujardi strain LPP7.

Author(s)

Maira Rodrigues de Camargo Neves

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-08-27

Examining board members

Carlos Eduardo Winter; Andréa Cristina Fogaça; Adriana Rios Lopes Rocha

• Advisor: Carlos Eduardo Winter

Abstract

Photorhabdus is a genus of Enterobacteriaceae, symbionts of Heterorhabditis, a genus of entomopathogenic nematodes. Among the enzymes secreted by P. luminescens TTO1 stands out PrtA, a metalloprotease that belongs to the subfamily of serralysins. PrtS, a protease capable of inducing a strong melanotic response from the insect, was identified in P. temperata Az29 but not in P. luminescens TTO1. In this work were detected and characterized biochemically few isolated proteases secreted by P. luminescens luminescens MN7. Two most prominent activities were detected: one with 50 kDa and one with 38 kDa. With the sequencing of the genome of MN7, we could confirm the presence in the genome of genes encoding proteins with high identity to PrtA and PrtS already described, with similar masses to those detected in our zimographies. These proteases are inhibited by specific inhibitors of metalloproteases. P. luminescens MN7 secretes a protease already described a few times, and other present only in P. temperata Az29. (AU)

• FAPESP's process: 11/16658-0 - Purification and characterization of some hydrolases secreted by Photorhabdus luminescens nssp. MN7
• Grantee: Maira Rodrigues de Camargo Neves
• Support Opportunities: Scholarships in Brazil - Master