Participation of GPER-1, a G-protein coupled estrogen receptor, in the estrogen-mediated neuroprotection of brain cortical primary cells in a glucose/oxygen deprivation model.

Author(s)

Dielly Catrina Favacho Lopes

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-08-22

Examining board members

Carolina Demarchi Munhoz; Niels Olsen Saraiva Câmara; Catarina Segreti Porto; Deborah Schechtman; Andréa da Silva Torrão

• Advisor: Carolina Demarchi Munhoz

Abstract

Estrogen is important to the development of neural networks. Thus, we investigated the cellular mechanisms related to neuroprotection through the rapid signaling mediated by GPER-1 in mixed culture and enriched neurons submitted or not to glucose/oxygen deprivation (OGD). We showed that cortical cell cultures express GPER-1 receptor and this are dispersed both in the cytosol and the nucleus. Our results showed that protection via estrogen signaling was dependent on the cellular composition. The lack of a signaling pathway GPER-1 before OGD increased cell death induced by OGD, suggesting that blocking of GPER-1 signaling pathway could be related to poor prognosis of ischemic lesions and G1 supplementation of culture media during deprivation and reperfusion attenuated these effects. In addition, our results point to the influence of glial cells as mediators of the neuroprotective role via non-nuclear estrogen signaling in this context of glucose/oxygen deprivation. (AU)