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| Author(s): |
Caio Jordão Teixeira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-09-23 |
| Examining board members: |
Carla Roberta de Oliveira Carvalho;
Roberto Barbosa Bazotte;
Fabio Bessa Lima
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| Advisor: | Carla Roberta de Oliveira Carvalho |
| Abstract | |
Deprivation of sex hormones in females contributes to the onset of metabolic and endocrine disorders due loss of estrogen. In this study the effect of castration and supplementation with DHEA on metabolic aspects in rats was evaluated. Ovariectomy increased adiposity and food consumption, decreased water consumption, besides promoting insulin resistance and glucose intolerance, and the use of DHEA reduced insulin sensitivity. Furthermore, castration led to decreasing of DHEA plasma levels, estradiol, VLDL-c and triacylglycerol, albumin, urea and creatinine, and DHEA treatment partially restored the concentration of estradiol in OVX+DHEA group. Finally, ovariectomy reduced the protein expression IRb, IRS1, PI3K and AKT in the liver, gastrocnemius muscle or heart. Indeed, decrease tyrosine phosphorylation status of pp185 (IRS1/2) was observed. Thus, we conclude that castration promoted insulin resistance and glucose intolerance associated with obesity, and in this model, the use of DHEA impairs insulin sensitivity in animals. (AU) | |
| FAPESP's process: | 12/14183-7 - Effect of supplementation with DHEA in the heart of rats with hypertension induced by castration and high fat diet |
| Grantee: | Caio Jordão Teixeira |
| Support Opportunities: | Scholarships in Brazil - Master |