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| Author(s): |
Elena Carolina Serrano Recalde
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2014-06-30 |
| Examining board members: |
Eneiva Carla Carvalho Celeghini;
Juliana Nascimento Bittar;
Luciano Andrade Silva
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| Advisor: | Eneiva Carla Carvalho Celeghini; Rubens Paes de Arruda |
| Abstract | |
The female reproductive tract soundness, as well as semen quality are factors that interfere in fertility. The aims of this study were to evaluate inflammatory uterine response and pregnancy rate in mares, considering uterine hemodynamics and endometrial cytology after artificial insemination (IA) with frozen semen of high and low quality. Two experiments were executed for the present study. For Experiment 1, fifteen mares were randomly distributed between four groups: CT - control: mimic of the procedure of IA (n=7), DIL: intrauterine infusion of skim milk semen extender (n=7), ALTA: IA with high quality semen (n=7), and BAIXA: IA with low quality semen (n=7). Uterine evaluation was done using transrectal Doppler ultrasonography by Spectral and Color-flow modes on seven moments: immediately before ovulation induction (TIO), immediately before AI (TIA), 2 (T2), 6 (T6), 12 (T12), 24 (T24) and 48 (T48) hours after IA. There were considered the numerical values of resistance index (RI) of uterine arteries and vascularity scores (EV) of uterine horns. Endometrial cytology was done at 6 h after IA. On Experiment 2, were used 12 mares which their cycles were distributed between the treatments: CT - control: only mimic of IA procedure (n=8), DIL: intrauterine infusion of semen extender (n=8), ALTA: IA with high quality semen (n=8), and BAIXA: IA with low quality semen (n=8). The experimental design was a Latin Square. Uterine evaluation was done using transrectal Doppler ultrasonography by Spectral and Color-flow modes on three moments: before ovulation induction (TIO), immediately before AI (TIA) and 6 hours (T6) after IA. Endometrial cytology was done at 6 h after IA. In this experiment it was considered the pregnancy rate comparing the cycles for groups ALTA e BAIXA. The pregnancy diagnosis was done 14 days after ovulation. It was used the Mixed Procedure (PROC MIXED) from SAS (Version 9.3) for statistical analysis and significant difference was considered when p≤0.05. On Experiment 1, no statistical difference was found between the groups for uterine hemodynamic values RI and EV. There was a significant increase of inflammatory cells on the mares inseminated with low quality semen, however it wasnt different from the group if mares inseminated with high quality semen. On Experiment 2, there was no statistical difference between the groups for RI values, but the uterine EV was higher for the groups inseminated with high and low quality when compared with the control group. There was no statistical difference on endometrial cytology. There was no statistical difference on pregnancy rate for the groups ALTA (81,82%) and BAIXA (54,55%). As a conclusion, the deposition of semen in the uterus causes an inflammatory process on the endometrium, and it could alter uterine dynamics detected by Doppler ultrasonography in mares. It does not exist significant difference on the inflammatory response between semen qualities. Low quality frozen-semen insemination does not alter pregnancy rate, but more studies are necessary in order to verify this difference. (AU) | |
| FAPESP's process: | 12/11353-9 - Pregnancy rate, inflammatory response and uterine hemodynamic after artificial insemination with cryopreserved semen evaluated by fluorescent probes |
| Grantee: | Elena Carolina Serrano Recalde |
| Support Opportunities: | Scholarships in Brazil - Master |