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| Author(s): |
Maíra Mello Rezende Valle
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-10-02 |
| Examining board members: |
Angelo Rafael Carpinelli;
Carla Roberta de Oliveira Carvalho;
Anna Karenina Azevedo Martins;
Fernando Neves Nogueira;
Kleber Luiz de Araujo e Souza
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| Advisor: | Angelo Rafael Carpinelli |
| Abstract | |
ROS production in pancreatic beta cells has been associated with the insulin secretion process but the mechanism by which glucose affects the redox state in these cells remains unknown. In order to address this issue, we evaluated the effect of 1 or 48 hours incubation of pancreatic beta cells with various glucose concentrations (2.8, 5.6, 8.3, 11.1, 16.7 and 20 mM). Glucose loading induced superoxide production by mitochondria and NADPH oxidase complex, and enhanced the antioxidant capacity by increasing GSH content and modulate expression of antioxidant enzymes. Glucose also promoted Rac1/Sod1 interaction that maintains NADPH oxidase activated. These cells however did not present redox endosomes, the redoxosomes, in response to glucose loading. These effects might be associated with the process of insulin secretion and pancreatic beta cell death. (AU) | |