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| Author(s): |
Marjorie Yumi Hasegawa
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2014-09-24 |
| Examining board members: |
Lilian Gregory;
Roberto Soares de Castro;
Edison Luiz Durigon;
Maria do Carmo Custodio de Souza Hunold Lara;
Huber Rizzo
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| Advisor: | Lilian Gregory |
| Abstract | |
Caprine Arthritis Encephalitis is an infectious, multisystemic disease caused by Caprine Arthritis Encephalitis virus, which belongs among the Small Ruminant Lentiviruses. Regarding the reproductive behavior in the transmission, the objective of the present study consists of experimentally evaluate the transmissibility of caprine lentivirus in goats and their offspring through the placenta and semen. To evaluate the influence of placentary transmission, five CAEV females were artificially inseminated with semen from CAEV-free buck. In order to evaluate the influence of transmission by semen, six CAEV-free females were artificially inseminated with semen from CAEV-free buck. The CAEV-Cork was added to fresh semen to obtain infecting titres for viral load of 105 TCID50/mL. As control group, two CAEV-free goats were artificially inseminated with semen from same buck without viral inoculum; and other two CAEV goats inseminated with the viral inoculum. The females were monitored during gestation until 15 days post-partum and the offspring, separated from their mothers, were monitored until 12 months old, using AGID, cELISA and nested-PCR techniques. The CAEV females presented positive results in AGID (87,10%), cELISA (88,71%) and nested-PCR (25,81%). The offspring presented negative results in both AGID and cELISA tests, however in nested-PCR technique, 7,14% of samples presented positive band. From the six CAEV-free females, four (66,67%) seroconverted with 18,46% positive samples at AGID, 49,23% at cELISA and none at nested-PCR. Anti-CAEV antibodies were detected 30 days after AI. Regarding to their offspring, 5,26% and 11,28% of samples presented positive results at AGID and nested-PCR, respectively. No sample was positive at cELISA. The CAEV-free females from control group had negative results at the three techniques, including their offspring; while the CAEV females inseminated with the viral inoculum have shown 61,90% of positive samples at AGID, 100% at cELISA and none at nested-PCR. Their offspring have only one sample positive at AGID and another at nested-PCR. The positivity found in offspring at nested-PCR may have a particular meaning in identify infected but seronegative animals, as in late seroconversion situations. However it is not possible to assume the CAEV transmission to offspring indisputably, though is not discarded the possibility of infection through semen and placenta in offspring with delayed seroconversion. Nevertheless it is possible the CAEV transmission by AI with infected semen in CAEV-free females. The viral load used in the present study was able to infect the females. Regarding the tests used, the cELISA detected seroconversion earlier than the AGID. The nested-PCR technique failed in detecting the infection before seroconversion in females. This study provided more information about the caprine lentivirus transmission concerning the reproductive aspect, using three different diagnostic methods. (AU) | |
| FAPESP's process: | 11/00773-4 - Study of the transmissibility of Caprine Arthritis Encephaliits virus by semen and placenta |
| Grantee: | Marjorie Yumi Hasegawa |
| Support Opportunities: | Scholarships in Brazil - Doctorate |