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| Author(s): |
Ademir Durrer Bigaton
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2015-01-23 |
| Examining board members: |
Fernando Dini Andreote;
Welington Luiz de Araujo;
Vania Maria Maciel Melo;
Luiz Fernando Wurdig Roesch;
Rodrigo Gouvêa Taketani
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| Advisor: | Fernando Dini Andreote |
| Abstract | |
Sugarcane is currently the most important culture of the State of São Paulo and has a prominent role among the crops in Brazil. Into the context of a better productivity with greater sustainability, the role of the microbial community present in the soil could have huge importance, aiding a better plant development, supplying it with nutrients or reducing the occurrence of diseases and pests. However, little is known about the microbial community existing in soils cultivated with sugarcane, where a knowledge of the spatial distribution of this community could be helpful to a better understanding of the processes that these organisms are involved. This project aimed to study in a biogeographic approach, the bacteria and archaea diversity in soils of sugarcane in the São Paulo State, focusing on the groups of archaea and bacteria. Analyses of a total of 285 soil samples, obtained in 10 producing distinct regions was performed using independent cultivation techniques such as quantification of total abundance by applying quantitative PCR (qPCR), analysis of the community structure by terminal restriction of length polymorphism (T-RFLP) and determination of its phylogenetic affiliation by high-throughput sequencing of 16S ribosomal genes. The results showed that the main drivers of these communities were the physical and chemical characteristics of the soil (pH, granulometry and organic matter). In addition, the results have shown that archaea community was influenced by management practices (mechanical harvest, vinasse and filter cake adding). Additionally, an unexpected relationship between the structures of these communities with the geographic distribution of the samples was observed. The results demonstrate the complexity of the community of bacteria and archaea along a spatial gradient, suggesting that future studies should consider a broader sampling of the distinct regions. This work supports upcoming studies that aim at developing agricultural practices exploring the soil microbiomes functionality. (AU) | |
| FAPESP's process: | 11/07343-5 - Bacterial and archaeal diversity in soils cultivated with sugarcane in the State of São Paulo: a biogeographic approach |
| Grantee: | Ademir Durrer Bigaton |
| Support Opportunities: | Scholarships in Brazil - Doctorate |