Ocurrence and diversity of multidrug-resistant gram-negative bactéria in public aquatic environments in southeastern Brazil.

Author(s)

Tatiane Nascimento

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-05-25

Examining board members

Nilton Erbet Lincopan Huenuman; Gabriel Padilla Maldonado; Maria Inês Zanoli Sato

• Advisor: Nilton Erbet Lincopan Huenuman

Abstract

Anthropogenic activities related to the massive use of antibacterial has contributed to the selection and spread of multidrug-resistant (MDR) into the aquatic environment. This study aimed to monitor the occurrence of MDR bacteria in public aquatic environments, in the state of São Paulo, characterizing genotypes of acquired resistance. Of the 50 gram-negative isolates, recovered of water samples, 70% exhibited a MDR profile. Indeed, blaCTX-M-2 (n= 5), blaCTX-M-9 (n= 1), blaCTX-M-15 (n= 2)-, blaKPC-2 (n= 3)-, qnrB (n= 1)-, oqxA (n= 3)-, oqxB (n= 3)- and aac(6)-1b-cr (n = 7) genes were identified. Noteworthy is the first the detection of KPC-2-producing Acinetobacter calcoaceticus. Aquatic environments, with public access, may be important sources for the dissemination and/or transmission of a wide variety of MDR bacterial species to both humans and associated ecosystems. Moreover, the presence of endemic genotypes suggests contamination by domestic and/or hospital sewage. (AU)

• FAPESP's process: 12/23588-0 - Prevalence and diversity of multidrug-resistant Gram-negative bacteria in public aquatic environment and drinking water sources in São Paulo State: clinical and environmental impact
• Grantee: Tatiane Nascimento
• Support Opportunities: Scholarships in Brazil - Master