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| Author(s): |
Carlos Andrés Fajardo Gómez
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-05-26 |
| Examining board members: |
Luiziana Ferreira da Silva;
Gabriel Padilla Maldonado;
Thatiane Teixeira Mendonça
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| Advisor: | Luiziana Ferreira da Silva |
| Abstract | |
To evaluate the possibilities of improving the productivity of PHA production from xylose, evolutionary engineering techniques were applied to B. sacchari to select cells with maximum specific growth rates (max) higher than the wild type. Metabolic flux analysis was also performed to evaluate the fluxes through central pathways and the possibility of further improvements by modifying fluxes rates. The evolved strain reached a max of 0.24 ± 0.01 h-1 at the end of the evolutionary process. Strains were submitted to bioreactor experiments. A metabolic network of the strain was usedn to determine the possible distribution of metabolic fluxes. A total of 19 elementary modes were obtained. It was concluded that the metabolism of xylose occurred mostly by VP along with the ED. The ED pathway has the major activity going on in a cyclic way. It was also performed a 13C labeled xylose assay, in which it was possibly to confirm the obtained results from the metabolic flux analyses. (AU) | |
| FAPESP's process: | 11/15405-0 - Development of a process to produce polyhydroxybutyrate from sugarcane bagasse derivatives using techniques of evolutionary engineering and bioprocess |
| Grantee: | Carlos Andres Fajardo Gomez |
| Support Opportunities: | Scholarships in Brazil - Master |