Evaluation of cardiac effects of angiotensin 1-7 in an animal model of hyperthyroidism.

Author(s)

Nathalia Senger

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-08-17

Examining board members

Maria Luiza Morais Barreto de Chaves; Jose Geraldo Mill; Luciana Venturini Rossoni

• Advisor: Maria Luiza Morais Barreto de Chaves

Abstract

The aim of this study is to verify if Angiotensin1-7 (Ang1-7) may influence the cardiovascular effects induced by thyroid hormone. Wild or Transgenic rats that constitutively overexpress Ang1-7 (TGR-L3292) received intraperitoneal injections of T3 (7µg/100g/day) for 14 days in order to develop hyperthyroidism. Cardiac morphology, function parameters and RAS components were evaluated in the heart. High levels of Ang1-7 attenuated cardiac hypertrophy and hemodynamic parameters induced by T3. Ang1-7 treatment improved the cardiac function of hyperthyroid animals. Moreover, high levels of T3 increased Angiotensin II levels, AT1 receptor and ACE2 activity in the heart. Anti-hypertrophic effects of Ang1-7 were also observed in vitro. Ang1-7 treatment (1000nM) prevented the increase in cell surface area in primary cultures of neonatal cardiomyocytes treated with T3 (10nM) for 24 hours. For the first time, cardioprotective actions of Ang1-7 were observed in the heart of hyperthyroid animals. (AU)

• FAPESP's process: 13/16348-6 - Evaluation of therapeutic role of Angiotensin 1-7 in the prevention of the cardiovascular effects induced by thyroid hormone in Wistar rats
• Grantee: Nathalia Senger
• Support Opportunities: Scholarships in Brazil - Master