Aerobic Exercise decreases NAFLD, but promotes liver inflammation in PPAR-alpha knockout mice via PPAR-gamma inhibition.

Author(s)

Helena Angelica Pereira Batatinha

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-09-24

Examining board members

José Cesar Rosa Neto; Patricia Chakur Brum; Fernanda Ortis

• Advisor: José Cesar Rosa Neto

Abstract

NAFLD is one of the main liver diseases. Studies have shown the beneficial effects of exercise on reverse NAFLD. We verify whether exercise improve insulin resistance, liver inflammation and steatohepatitis caused by a high fat diet (HF) and whether PPARα is involved in these actions. C57BL6 wild type (WT) and PPAR-α knockout (KO) mice were fed with a standard (SD) or HF during 12 weeks and trained on a treadmill during 8 weeks, half of KO trained animals received 15mg/kg/day of rosiglitazone. HF diet increased TAG in the liver and peripheral insulin resistance leading to NAFLD. Exercise reduced all this parameters in both animals genotype. NAFLD was not associated with inflammation, however KO mice when trained presented an inflammatory response that was caused by a decrease on PPARγ. When these mice were treated with rosiglitazone, they presented decrease on inflammatory cytokines as well as improvement on insulin sensitivity. Exercise improved the damage caused by a HF independently of PPARα and the absence of PPARα together with exercise leads to decrease on PPARγ expression and an inflammatory response, which was attenuated by rosiglitazone administration. (AU)

• FAPESP's process: 13/10861-3 - The envolviment of the PPAR alpha nuclear receptor, on physical exercise protector effects against inflammation ANS hepatic steatosis induced for hGH fat diet
• Grantee: Helena Angélica Pereira Batatinha
• Support Opportunities: Scholarships in Brazil - Master