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| Author(s): |
Heloisa Berti Gabriel
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-10-09 |
| Examining board members: |
Alejandro Miguel Katzin;
Daniel Youssef Bargieri;
Pedro Vitor Lemos Cravo;
Danilo Ciccone Miguel;
José Franco da Silveira Filho
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| Advisor: | Alejandro Miguel Katzin |
| Abstract | |
Malaria is one of the main widespread human parasites. The lack of an effective vaccine and the problem of drug resistance haves contributed to the delay of the control solution of this infection. The search for new biological targets has focused in part on the understanding of metabolic pathways. In P. falciparum, identified the biosynthesis of the two forms of vitamin K (phylloquinone and menaquinone). In the MEP pathway were characterized two important bifunctional enzyme, farnesyl diphosphate synthase/geranylgeranyl diphosphate synthase (FPPS/GGPPS) able to form farnesyl diphosphate and geranylgeranyl diphosphate and octaprenyl pyrophosphate synthase/phytoene synthase (OPP/PSY) responsible for the biosynthesis of isoprenic side chains attached to the benzoquinone ring of ubiquinones, but also forms the first carotene in the carotenoid pathway. This project aims to characterize the MenA gene from the MQ biosynthesis, determine the localization of FPPS/GGPPS and investigate the importance of OPP/PSY and FPPS/GGPPS in intra-erythrocytic cycle of P. falciparum. (AU) | |
| FAPESP's process: | 10/19518-1 - Functional characterization of farnesyl pyrophosphate synthase (FPPs) and 1,4-dihydroxy-2-naftoato preniltransferase (MenA) respectively involved in the isoprenoid pathway and the vitamin K during the intra-erythrocytic development of P. falciparum |
| Grantee: | Heloisa Berti Gabriel |
| Support Opportunities: | Scholarships in Brazil - Doctorate |