Chromatographic evaluation and sorption studies and toxicity in earthworms of deltamethrin, glyphosate and aminomethylphosphonic acid

Author(s)

Fernanda Benetti

• Document type: Doctoral Thesis
• Press: São Carlos.
• Institution: Universidade de São Paulo (USP). Instituto de Química de São Carlos
• Defense date: 10 dez. 2015

Examining board members

Maria Olimpia de Oliveira Rezende; Irineu Bianchini Junior; Wilson Tadeu Lopes da Silva

• Advisor: Maria Olimpia de Oliveira Rezende

Abstract

In Brazil it is known that the use of agricultural inputs to improve productivity has been increasing. Among these inputs, we can highlight the use of pesticides. This work developed and validated a methodology for deltamethrin analysis (a pyrethroid used as insecticide) by HPLC/UV. These samples were evaluated in the presence of glyphosate (a substituted glycine used as herbicide) and its metabolite, AMPA, by GC/MS. These methodologies were applied to analyze real samples of water, soil and sediment in São Carlos in order to evaluate the contamination by these xenobiotics. To elucidate the potential contaminant and leaching of these, we also evaluated the interaction with humic acids, and adsorption studies were performed in red latosol where there was a great influence of organic matter on xenobiotics retention in the soil. So as to propose a method for remediation of soils in cases of contamination by shedding of commercial formulations in soil, three toxicity tests (mortality, biomass and reproduction) involving Eisenia foetida earthworms with the addition of 3% of hummus were performed. All the study was developed in compliance with the Quality Management concepts covered in ISO 17025. (AU)

• FAPESP's process: 11/22651-8 - Evaluation of contamination of soil impacted with organic xenobiotic on great economic expression cultures and its remediation with vermicomposting
• Grantee: Fernanda Benetti
• Support type: Scholarships in Brazil - Doctorate