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Structural and functional studies of an endoglucanase from Phanerochaete chrysorporium belonging to the glycoside hydrolase family 45

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Author(s):
Marina Paglione Ramia
Total Authors: 1
Document type: Doctoral Thesis
Press: São Carlos.
Institution: Universidade de São Paulo (USP). Instituto de Física de São Carlos
Defense date:
Examining board members:
Igor Polikarpov; Ana Paula Ulian de Araujo; Artur Torres Cordeiro; Carlos Alberto Labate; Cristina Paiva de Sousa
Advisor: Igor Polikarpov
Abstract

The importance of the study of the cellulases is not limited to generating significant scientific knowledge, since these enzymes represents an enormous potential in biotechnology. This is partly because cellulose is the most abundant molecule in nature and provides a wide range of products and sustainable process. Many cellulases families have been well characterized, while others still remain unknown. Among them, the glycoside hydrolase family 45 is the least well characterized both structurally and functionally, between fungal cellulases. It was recently proposed the subdivision of this family into three subfamilies, with structural information available only for subfamily A. In this work, we report the chrystallographic structure of the recombinant endoglucanase from Phanerochaete chrysosporium (PcCel45A), the first GH45 subfamily C and its complex with cellobiose at 1.4 Å and 1.7 Å respectively. The PcCel45A is a single domain enzyme, which has a β-barrel structure with the overall shape resembling an anchor. The active site of the enzyme has a long cleft on the surface, being remarkably different from those members of subfamily A, and the catalytic aspartate responsible for acting as proton acceptor (Asp10) is not present. Additionally, the chrystallographic structure of this enzyme has shown more similarity with β -expansins (plant proteins) and lytic transglycosylase (proteins that cleave the peptidoglycan of bacteria) than others representants of family 45, which makes it more singular. For a better understanding of its function, we perform pontual mutations in the main residues from active site. The Asp121, known for acting as proton acceptor in the inversion reaction of others enzymes, proved to be essential for the enzyme activity, while others conserved residues as Tyr25, Trp161 and Asp92 affected but not annihilated the enzyme activity, leaving approximately 20%, 50% and 10% of the native enzyme activity. (AU)

FAPESP's process: 11/19100-0 - Functional and structural studies of enzyme class endoglucanases GHF45 aiming for application in biomass hydrolysis
Grantee:Marina Paglione Ramia
Support type: Scholarships in Brazil - Doctorate (Direct)