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| Author(s): |
Cláudia de Lázzari Almeida
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2015-08-21 |
| Examining board members: |
Alexandra Christine Helena Frankland Sawaya;
Marcia Ortiz Mayo Marques;
Paulo César Pires Rosa
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| Advisor: | Alexandra Christine Helena Frankland Sawaya |
| Abstract | |
The use of medicinal plants and phytotherapic medicine is being increasingly stimulated by government policies at all levels. Syrup based on Mikania glomerata Spreng.is presently furnished by the Brazilian Health System (SUS) as medicine for cough and bronchitis. However, variations in the cultivation conditions of medicinal plants may result in different concentrations of active components, affecting the safety, quality and efficiency expected of herbal medicines. Few studies regarding the cultivation of the two species of interest as medicinal plants, Mikania glomerata e Mikania laevigata Schultz, were found. To this moment, the fluctuations in the concentrations of secondary metabolites in these species due to environmental variations have not been satisfactorily evaluated. As both species are found in the 1st Brazilian Phytotherapic Formulary, and apparently may be used indiscriminately, they shall be studied in parallel. We evaluated how the secondary metabolites are influenced by variations in the following cultivation conditions: temperature, luminosity and mechanical damage. After the treatments, the extracts of the leaves was evaluated by liquid chromatography-mass spectrometry, following the differences in the concentration of their chemical marker, coumarin, and the profile of the other components. The results show significant influence of treatments on various secondary compounds. After mechanical damage, both species showed a decrease in most of the compounds in the first hour, and subsequent increase after 24 hours of the damage. Despite the coumarin be considered the chemical marker for both species, it was found in small concentrations, and even absent in M. glomerata. In response to the treatment of light, we observed a significant increase of phenolics compounds such as chlorogenic acid and dicaffeoylquinic acid in plants grown in full sun light. In response of changes in temperature, we observed an increase of various phenolic compounds, including coumarin, in plants kept temperatures of 12°C and 30°C. Observing the chemical difference between the two species and the difference response of each one for the variation of cultivation conditions, we concluded that they need to be differentiated and require standardization of culture conditions for a safe and effective therapeutic use (AU) | |
| FAPESP's process: | 13/15962-2 - Secondary metabolites of two species of guaco (Mikania glomerata Sprenguel and Mikania laevigata Schultz) grown under varied conditions |
| Grantee: | Claudia de Lazzari Almeida |
| Support Opportunities: | Scholarships in Brazil - Master |