Functional characterization of CD100 / SEMA4D in macrophage infection by Leishmania (Leishmania) amazonensis.

Author(s)

Mariana Kolos Galuppo

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-02-19

Examining board members

Beatriz Simonsen Stolf Carboni; Selma Giorgio; Lourdes Isaac; Ana Paula Lepique; Renato Arruda Mortara

• Advisor: Beatriz Simonsen Stolf Carboni

Abstract

Leishmaniasis is caused by trypanosomes of the genus Leishmania that preferentially infect macrophages. Several factors influence the form and severity of the disease: the species of Leishmania and the host immune response. Considering the importance of the activation of macrophages in infection, potential role of CD100 in the modulation of macrophage activation and our previous data that CD100 soluble (sCD100) increase the infectivity of the parasite, we intend to characterize the effect of CD100 in infection with Leishmania (L.) amazonensis. We found that both promastigotes and amastigotes, are most infectious in the presence of sCD100 and the CD72 receptor is responsible for the increased infection. In vitro experiments indicated similar infection rate of macrophages to CD100 knockouts and wild type, but interestingly, the infected knockout animals developed significantly smaller lesions than wild type suggesting that sCD100 present in other cells may influence lesion formation. (AU)

• FAPESP's process: 12/15048-6 - Analysis of the participation and localization of CD100 during infection of macrophages by Leishmania (Leishmania) amazonensis
• Grantee: Mariana Kolos Galuppo
• Support Opportunities: Scholarships in Brazil - Doctorate