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Effect of seminal plasma on motility characteristics and fertility of cryopreserved boar spermatozoa

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Author(s):
Mariana Andrade Torres
Total Authors: 1
Document type: Master's Dissertation
Press: Pirassununga.
Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
Defense date:
Examining board members:
André Furugen Cesar de Andrade; Nestor Gaston Sepulveda Becker; Carlos Henrique Cabral Viana
Advisor: André Furugen Cesar de Andrade
Abstract

Boar semen cryopreservation is still a challenge due to the extension of cold shock damage. Boar spermatozoa arising from sperm-rich ejaculate fraction are reported to have a more stable plasma membrane, more resistant to cold shock and premature acrosome reaction than spermatozoa from the whole ejaculate. Thus, seminal plasma arising from whole sperm-rich fraction can increase cryotolerance of boar spermatozoa, and in other domestic species it has the ability to reverse cryopreservation damage. However, the majority of actual sperm evaluation techniques include a single sperm parameter, which makes the sperm characterization of a single population difficult. In this context, this work was performed to validate a sperm flow cytometry fourfold coloration technique for the simultaneous evaluation of plasma and acrosomal membrane integrity and mitochondrial membrane potential. Furthermore, we evaluate the seminal plasma arising from whole sperm-rich fraction effects on sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential, tyrosine phosphorylation, membrane fluidity and peroxidation, fertility and early pregnancy rate. The proposed technique is able (R2 = 0.9356; p < 0.01) to simultaneous detection of plasma and acrosomal membrane integrity and high mitochondrial membrane potential (IPIAH). Seminal plasma arising from whole sperm-rich fraction is able to improve (p < 0.05) total and progressive motility. Furthermore, seminal plasma arising from whole sperm-rich fraction maintenance/addition was not able to improve (p > 0.05) the IPIAH sperm population, tyrosine phosphorylation, membrane fluidity and peroxidation. However, these sperm characteristics need to be carefully interpreted, once, membrane fluidity and peroxidation could be influenced (p < 0.05) by centrifugation. Fertility and early pregnancy rate we not improved (p > 0.05) by seminal plasma addition. Therefore, we concluded that sperm fourfold coloration can be used to evaluate simultaneously plasma and acrosomal membranes integrity and mitochondrial membrane potential and that whole sperm-rich fraction seminal plasma can be beneficial to post-thawed sperm physiology, thereby improving boar semen cryopreservation. (AU)

FAPESP's process: 13/08070-8 - Effects of seminal plasma on motility characteristics and fertility of cryopreserved boar sperm
Grantee:Mariana Andrade Torres
Support Opportunities: Scholarships in Brazil - Master