Influence of overweight and maternal obesity on immunological, biochemical and hormonal parameters of blood and human colostrum

Author(s)

Mahmi Fujimori

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Saúde Pública (FSP/CIR)
• Defense date: 2016-08-19

Examining board members

Luiz Carlos de Abreu; Adenilda Cristina Honorio França; Eduardo Luzia França; Claudio Leone; Viviane Gabriela Nascimento

• Advisor: Luiz Carlos de Abreu

Abstract

Introduction: Maternal obesity during pregnancy is characterized by an exaggerated state of inflammation compared to mothers without excess weight. There is evidence that a delicate balance of proinflammatory and anti-inflammatory compounds is transferred from mother to infant via breastfeeding. It is unknown whether the maternal systemic inflammation that is present in obese women influences the cytokine levels of colostrum. Objective: To evaluate the cytokine profile Th1, TH2, TH3, TH17 of blood and colostrum of overweight and obese mothers, and verify biochemical, hormonal and immunological parameters of human blood and colostrum. Methods: Were evaluated 68 mothers that were divided according to their BMI status: lean (n=25), overweight (n=24) and obese (n=19). The concentrations of glucose, triglycerides and total cholesterol were measured by colorimetric enzymatic method, and total proteins by colorimetric method. Fat content and calories of colostrum were obtained by Creamatocrit method. The hormones leptin and adiponectin were measured by ELISA technique. Immunoglobulins and complement protein (C3 and C4) and PCR by Turbidimetric method. Concentrations of cytokines were assessed by flow cytometry. Results: We observed higher glucose levels, fat content, calories and IgA in colostrum of obese mothers. Serum concentrations of triglycerides, cholesterol, IgA, CRP, C3 and C4 complement proteins were significantly higher in obese mothers. No significant differences in concentrations of the cytokines IL-2, IL-4, IL-10 and IL-17 were found between the groups studied in maternal blood and colostrum. The obese mothers had the highest serum levels of IL-6 and TNF- and minor concentrations of TGF- in colostrum. We found higher leptin levels in maternal blood and colostrum of obese mothers. Obese mothers had the lowest adiponectin levels in blood. In colostrum there was an increase in 5 the concentration of this hormone. Conclusions: The results of this study suggest that maternal obesity changes biochemical, immunological and hormonal components of human colostrum leading to repercussions in its role in the development of the newborn. (AU)

• FAPESP's process: 12/16662-0 - INFLUENCE OF OVERWEIGHT AND OBESITY ON MATERNAL IMMUNOLOGICAL,BIOCHEMICAL AND HORMONAL PARAMETERS IN MOTHER-CHILD RELATIONSHIP.
• Grantee: Mahmi Fujimori
• Support Opportunities: Scholarships in Brazil - Doctorate