Histological and histomorphometric evaluation of the bone regeneration with the use of chitosan, collagen and hydroxyapatite composites as a bone substitute in bone defect induced in the tibia of sheep

Author(s)

Nicole Fidalgo Paretsis

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
• Defense date: 2016-05-23

Examining board members

André Luis do Valle de Zoppa; Rodrigo Romero Corrêa; Neimar Vanderlei Roncati

• Advisor: André Luis do Valle de Zoppa

Abstract

Bone defects are commonly found in veterinary medicine through different disease such as trauma, especially in large animals. Frequently it is necessary to assist this tissue repair for better regeneration. Biomaterials in general are designed to this function. In order to understand the bone cells in the presence of bone substitutes, tests in vivo are essential for these studies. The quantity and quality of new tissue formation can be assessed by observation of tissue morphofunctional unit. Currently, in orthopedic studies sheep are considerated animals of choice for experimental development. Bone defects were performed experimentally in sheep and were implanted chitosan, hydroxyapatite and collagen biomaterials. After 60 days, biopsy was performed at the site of tissue repair and the new tissue formation was described, evaluated and compared by histomorphometry and electronic transmission microscopy. After hystomorfometry analysis there was no difference between control group and biomaterial group, but when compare with pre-existent tissue, the values are smaller. In descriptive analysis was observed intimate contact with biomaterial and new tissue formation suggesting biocompatibility. It observed the pattern of tissue repair in control group and biomaterial group did not differ in the architecture tissue. However, in the biomaterial group was identified presence of the biomaterial in the specimen. All these data suggest that the biomaterial study has the potential to assistant in orthopedic routine of large animals. (AU)

• FAPESP's process: 14/16670-8 - Histological and histomorphometric evaluation of chitosan, collagen and hydroxyapatite composites as bone graft substitute in a sheep tibial defect
• Grantee: Nicole Fidalgo Paretsis
• Support Opportunities: Scholarships in Brazil - Master