Effects of experimental infection with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in mice pregnancy pattern and inflammatory markers.

Author(s)

Marcela Yang Hui Zi

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-09-14

Examining board members

Marcia Pinto Alves Mayer; Ângela Silva Barbosa; Luciana Saraiva

• Advisor: Marcia Pinto Alves Mayer

Abstract

This study aims to determine the effect of experimental infection by periodontal pathogens on pregnancy. Periodontitis was induced with P. gingivalis following mating. Infection was performed by A.actinomycetemcomitans IV in pregnant mice. On the 16th day of pregnancy, the animals were sacrificed. The test group Pg showed lower weight and reduced fetal weight. Pg was detected in the test group in fetuses (8/27), placentas (9/27) and amniotic fluid (1/9). There was no difference of cytokine in gum and serum. Placental IL-1β, INF-γ, IL-10, GM-CSF and IL-12 were higher in the test compared to the control. The Aa group presented bacteria fetuses (11/14), placenta (11/14) and amniotic fluid (1/6). There was no difference in parameters related to pregnancy and serum cytokines between test and control, but the placental levels of TNF-α, IL-4 and IL-6 were higher in the test group. The data suggest the ability of periodontal pathogens overcome the placental barrier, colonize and increase the production of inflammatory mediators in the fetoplacental unit. (AU)

• FAPESP's process: 14/10549-2 - Effects of experimental periodontal disease induced by Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in the fetal-placental unit and systemic inflammatory markers
• Grantee: Marcela Yang Hui Zi
• Support Opportunities: Scholarships in Brazil - Master