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| Author(s): |
Bruna Bizzarro
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-10-14 |
| Examining board members: |
Anderson de Sá Nunes;
Karina Ramalho Bortoluci;
Silvia Beatriz Boscardin;
Alexandra Ivo de Medeiros;
Ricardo Weinlich
|
| Advisor: | Anderson de Sá Nunes |
| Abstract | |
In the present work, we described in the saliva of Aedes aegypti mosquito an activity of cell death induction for lymphocytes characterized by fast phosphatidylserine exposure in cell surface, incorporation of propidium iodide, cell shrinkage, loss of CD62L expression, and selectivity for naïve lymphocytes. The molecule responsible for this activity was identified by fractionation of mosquito salivary gland extract (SGE) by HPLC and mass spectrometry. The messenger RNA that codifies this molecule is abundantly present in A. aegypti females but not in males, suggesting its role in blood-feeding. RNA interference was employed to silence the gene responsible for this activity in A. aegypti females and the SGE of these mosquitoes lost the ability to induce lymphocyte death, confirming that this is the molecule responsible for the activity. In conclusion, this work identifies a new molecule from A. aegypti saliva with a fast, potent and selective activity on lymphocytes. (AU) | |
| FAPESP's process: | 12/11816-9 - Immunofunctional characterization of a lymphocyte inhibitor in Aedes aegypti saliva |
| Grantee: | Bruna Bizzarro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |