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Effect of interesterified fats intake on inflammatory and metabolic pathways in the liver and adipose tissue in ldlr- ko mice

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Author(s):
Maria Silvia Ferrari Lavrador
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Faculdade de Medicina
Defense date:
Examining board members:
Ana Maria Pita Lottenberg; Luciani Renata Silveira de Carvalho; Dennys Esper Corrêa Cintra; Márcio Corrêa Mancini
Advisor: Ana Maria Pita Lottenberg
Abstract

Background: interesterified fats, rich in saturated fatty acids, have been used by the food industry as a substitute for trans fatty acids. The main saturated fatty acids used in the interesterification process are palmitic and stearic, which may, respectively, have deleterious effects or be cardiovascular and metabolic neutral. Objetive: to evaluate the effect of interesterified fats containing palmitic or stearic acids on signaling pathways involved in the hepatic and adipose tissue lipid metabolism. Methods: Weaning male LDLr-KO mice were fed a high-fat diet (40% energy as fat) containing polyunsaturated (PUFA), palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR) or stearic interesterified (STEAR INTER) fats for 16 weeks. Body composition, liver fat content, total plasma cholesterol, triglycerides, glucose and insulin concentrations were determined. Gene expression involved in lipid metabolism and inflammation process in liver and white adipose tissue was determined by quantitative RT-PCR. Furthermore, amount of proteins and activated proteins were measured by Western blot. Hepatic and adipose tissue inflammatory cells infiltration as well as liver collagen content were determined by eosin and hematoxylin and Sirius Red staining, respectively. Results: Interesterified process did not alter plasma biochemical parameters and amounts of TC and TG in hepatic tissue. However, both interesterified fats brought on a higher liver collagen content and JNK phosphorylation. Additionally, STEAR INTER group developed NASH associated with a higher neutrophil infiltration. On the other hand, PALM INTER induced a higher adipose tissue expansion together with the enlargement of adipocytes, conditions associated with the activation of upstream NFkB signaling pathway as observed for IKK phosphorylation, contributing to higher TNFalpha protein content. Conclusions: Interesterified fats containing stearic acid induced NASH and enriched with palmitic acid trigger on steatosis with fibrosis as well as hypertrophy and inflammatory signaling activation in the adipose tissue in LDLr-KO mice (AU)

FAPESP's process: 11/24083-7 - Effect of interesterified fat consumption in the inflammatory and metabolic pathways in the liver and adipose tissue of LDLr-KO mice.
Grantee:Maria Silvia Ferrari Lavrador
Support type: Scholarships in Brazil - Doctorate