Effects of fructose consumption during gestation and lactation and its repercussion on postnatal life: a metabolic programming study in male rats.

Author(s)

Renata Juliana da Silva

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2017-04-05

Examining board members

Jackson Cioni Bittencourt; Dennys Esper Corrêa Cintra; Rui Curi; Fernando Salvador Moreno; Silvana Auxiliadora Bordin da Silva

• Advisor: Jackson Cioni Bittencourt; Marcelo Macedo Rogero

Abstract

INTRODUCTION: Maternal exposure to fructose during gestation and lactation can contribute to the development of chronic diseases in the adult life of offspring. OBJECTIVE: To study the effects of ingestion of fructose during gestation and lactation on postnatal life with respect to metabolic changes, gene expression and feeding behavior in male rats. METHODS: Male Sprague-Dawley rats from mothers fed with control rat chow (AIN-93) and from mothers fed a high fructose diet (60%). Both isocaloric, during gestation; lactation and gestation/lactation. After weaning, the offspring were fed with control rat chow until the 91st days of life. The following parameters were analyzed: biometric, dietary, metabolic for glycaemia, peripheral insulin sensitivity, leptin and insulin (serum and signaling pathways), neuropeptides involved in food behavior control, and hepatic deposition of fat. RESULTS: Reduction in the Lee index at birth and hypoleptinemia at 90 days of age (only in the offspring that the mothers consumed fructose during pregnancy), glucose intolerance in postnatal life since with changes in insulin sensitivity in adult life, hyperinsulinemia, dyslipidemia, hepatic deposition of GAD, hemodialysis of altered eating behavior at 14 days of age). CONCLUSION: The offspring from mothers who consumed fructose rat chow during gestation and lactation have a possible epigenetic mark that may contribute to the impairment in the production and action of insulin, leading to changes in hepatic glucose and lipid metabolism homeostasis, leading to hepatic fat deposition and alterations in the lipid profile, with reduction of leptinemia in adult life, and in the central hedonic control of dietary behavior only at the 14 days postnatal life. (AU)

• FAPESP's process: 12/04231-4 - EFFECTS OF CONSUMPTION OF FRUCTOSE DURING PREGNANCY AND LACTATION AND ITS IMPACT ON LIFE AFTER CHRISTMAS: A STUDY OF METABOLIC PROGRAMMING IN MALE RATS
• Grantee: Renata Juliana da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate