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| Author(s): |
Cristhiane Favero de Aguiar
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2017-04-07 |
| Examining board members: |
Niels Olsen Saraiva Câmara;
José Antonio Tavares de Albuquerque;
Alexandre de Castro Keller;
Eliana Faquim de Lima Mauro;
Pedro Manoel Mendes de Moraes Vieira
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| Advisor: | Niels Olsen Saraiva Câmara |
| Abstract | |
NKT cells comprise a distinct group of lymphocytes characterized by their reactivity to glycolipids presented by CD1d. In our work, we investigated the involvement of NKT cells in intestinal homeostasis using knockout (KO) mice and we also characterized NKT subtypes in the intestine. The absence of NKT cells in KO mice influenced the intestinal microbiota and homeostasis. KO mice had a decrease in the Firmicutes phyla, in the levels of fecal IgA and TGF-β in the intestine. These mice are also more susceptible to DSS-induced colitis, exhibiting worse survival, severe shortening of the colon and higher score. In wild type mice, the induction of the NKT10 subtype decreased intestinal inflammation, did not cause intense reduction of colon and reduced the frequency of NKT cells and the NKT17 subtype in the intestine. Our results indicate that intestinal NKT cells are important for intestinal homeostasis and microbiota composition. (AU) | |
| FAPESP's process: | 12/16794-3 - Influence of gut microbiota on NKT cells profile in an experimental model of colitis |
| Grantee: | Cristhiane Favero de Aguiar |
| Support Opportunities: | Scholarships in Brazil - Doctorate |