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| Author(s): |
Barbara Cristina da Silva Fernandes
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2016-02-02. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2016-01-19 |
| Advisor: | Carlos Ducatti; Juliana Célia Denadai |
| Abstract | |
The aim of this study was to trace the presence of animal by-products in commercial broiler farms feed using the methodology of stable isotope ratios of carbon and nitrogen (δ13C and δ15N) in the pectoral muscle and intestinal mucosa, and start a database of bromatological and isotopic values of feed ingredients with greatest interest in poultry production. Eight male birds were sampled randomly week-by-week in seven broiler farms of different poultry companies in the São Paulo State to obtain samples of pectoral muscle and intestinal mucosa. Were taken on the same day of sampling three random samples of feed, acquired from feeders of each commercial broiler unit. For the database were sampled corn, soybean, soybean meal and animal by-products obtained from reception and quality control departments of visited feed factories. All samples were analyzed using isotope ratio mass spectrometry, and feed was also evaluated for the presence of animal DNA by PCR analysis. The isotopic data of the samples were submitted to analysis of variance in each age collection, complemented by Tukey test, and the set of all data were analyzed by multivariate analysis of major and linear discriminant components. It was verified by PCR analysis in feed that only D and F companies used strictly vegetable diet throughout the period of raising birds. There was difference between isotopic values of feed, pectoral muscle, and intestinal mucosa of birds from D and F companies compared with others, which is most definitely for the δ15N from seven to 42 days old. The same result was obtained by multivariate analysis. Therefore, it was possible to differentiate the companies that used strictly vegetable diet from the others, demonstrating the ability to trace the presence of animal by-products in commercial broiler farms feed using the methodology of stable isotopes. (AU) | |
| FAPESP's process: | 13/04339-2 - COMMERCIAL BROILER CERTIFICATION THROUGH STABLE ISOTOPE TECHNIQUE (delta 13C and delta 15N) |
| Grantee: | Barbara Cristina da Silva Fernandes |
| Support Opportunities: | Scholarships in Brazil - Doctorate |