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Maintaining the balance of intestinal microbiota of broilers with probiotics after antibiotic

Grant number: 11/12998-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2012
Effective date (End): October 31, 2014
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:José Fernando Machado Menten
Grantee:Rafaela Pereira
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated scholarship(s):13/07571-3 - Effect of changes in the intestinal microbiota of layer-type chickens in resistance to enteric infections and immune function, BE.EP.DR


The aim of the study will evaluate the efficiency of addition of probiotics in broiler diets, maintenance of the structure of the intestinal microbiota following antibiotic therapy. In the essay will be housed chicks one day, and the trial period will be from 1 to 42 days old. The experimental design is fully randomized, with four treatments and nine replicates. The treatments are: Basal diet without probiotics; basal diet + probiotic; basal diet + antibiotic therapy at 21 days of age and basal diet + probiotic + antibiotics at 21 days old. For the determination of the performance variables, the animals are weighed at 7, 14, 21, 35 and 42 days old, and the rations will be provided and quantified the leftovers. At 26, 28 and 30 days old will be used four replicates per treatment for analysis of gut microbiology. Three birds from each boxing will be sacrificed to collect material from the mucosa of the ileum and cecum. The composite sample of DNA will be extracted and the specific fragments of V1-V3 region 16S rDNA microorganisms representing the domain Bacteria will be amplified. Will be used DGGE technique, which assesses changes in the intestinal community structure, and quantitative PCR to quantify the main components of the intestinal tract of broilers as Bifidobacteria, Lactobacillus and Clostridium. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PEREIRA, Rafaela. Dietary probiotic in broiler chickens submitted to antibiotic therapy: performance and intestinal microbiota. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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