Intracellular and extracellular vesicles miRNAs profile during neural differentiation of pluripotent stem cells.

Author(s)

Lilian Cruz

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2017-04-05

Examining board members

Marilene Hohmuth Lopes; Patricia Cristina Baleeiro Beltrao Braga; Ricardo José Giordano; Enrique Mario Boccardo Pierulivo; Chao Yun Irene Yan

• Advisor: Marilene Hohmuth Lopes; Raphael Bessa Parmigiani

Abstract

Stem cells sense and process multiple signals in their microenvironment, which can exert influences that regulate cell fate and function in a time spatial manner. In this context, the stem cells can exert their biological role transferring genetic information and altering the genetic expression of target cells through extracellular vesicles (EVs). MicroRNAs (miRNAs), a class of small non coding RNAs, can be found in those EVs and are considered effective molecules in the control of neurodevelopment and differentiation by regulating key genes in a time specific manner. However, little is known about how the cell differentiation influences the miRNAs content released through EVs, and how these molecules function in the microenvironment of each phase of neural commitment. Thus, the purpose of this study was to analyze the intracellular and EVs miRNAs profiles involved in the dopaminergic differentiation of pluripotent stem cells in attempt to identify possible targets regulated by miRNAs as a mechanism of specific neural fate decision. (AU)

• FAPESP's process: 13/22078-1 - Profile of extracellular vesicles miRNA in the neuronal differentiation of murine embryonic stem cells
• Grantee: Lilian Cruz
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)