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| Author(s): |
Caroline Mantovani da Luz
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2016-12-15 |
| Examining board members: |
Rui Alberto Ferriani;
Fernando Marcos dos Reis
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| Advisor: | Paula Andrea de Albuquerque Salles Navarro |
| Abstract | |
The endometriosis related infertility mechanisms still remain to be elucidated, although several studies propose a deleterious effect of this disease on oocyte quality. However, the oocyte analysis in patients with endometriosis is not routinely feasible, since human oocytes are rarely donated to researches and their application in invasive techniques precludes their use in reproduction procedures. In this context, evidences suggest that cumulus cells (CC) could be used as indirect biomarkers capable of predicting oocyte quality. Thus, through an unprecedented study, we aimed to determine the differential transcripts profile in CC of infertile women with and without advanced endometriosis and its different stages, undergoing controlled ovarian stimulation for intracytoplasmic sperm injection. Therefore, we performed a case-control study, which analyzed by RNA next generation sequencing (RNA-Seq), 3 groups (control, endometriosis III/IV without endometrioma and endometriosis III/IV with endometrioma), with 3 pools of 3 patients each. The comparison of top deregulated genes among groups of advanced endometriosis and control patients show us altered genes associated with oxidative pathways, acetylation and ubiquitination process, aside from genes related to cholesterol and estradiol regulation. These data elucidated that CC of infertile woman with advanced endometriosis carried essential molecular alteration linked with follicular and gametic development. Through enrichment of the top deregulated genes, we can point out the potential pathways altered in this process toward oocyte commitment and infertility in these patients. (AU) | |