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In situ analysis of Er:YAG laser irradiation to control the progression of erosive and abrasive lesions on tooth enamel

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Author(s):
Renata Siqueira Scatolin
Total Authors: 1
Document type: Doctoral Thesis
Press: Ribeirão Preto.
Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC)
Defense date:
Examining board members:
Silmara Aparecida Milori Corona; Maria Cristina Borsato; Edson Alves de Campos; Aline Evangelista de Souza Gabriel; Paulo Sérgio Quagliatto
Advisor: Silmara Aparecida Milori Corona
Abstract

This study, composed of two in situ experiment, evaluated the effect of Er:YAG laser irradiation to control the progression of erosive and abrasive lesions on enamel. Bovine incisors were sectioned (5x3x2.5mm), flattened and polished. The enamel slabs (KHN = 330 ± 10%) with one fourth of the surface covered with resin composite (reference area) were submitted to initial erosion-like lesion formation with 1% citric acid (pH 2,3), 5 min, 2x/day for 2 days. In the first experiment, 56 slabs were divided into two groups according to the surface treatment: irradiated with Er:YAG laser (5,2 J / cm2, 2 Hz, 85 mJ, 10 s) and non-irradiated, and subdivided according to the type of wear: erosive (citric acid) and non-erosive (deionized water). The challenges were performed by immersing the devices in 100 mL citric acid or deionized water, 3x/day, randomly and independently. Enamel loss was determined by an optical 3D profilometer after: initial erosion, surface treatment and intraoral phase. The average of structure loss was verified by three readings of each experimental area comparing with reference area. Data was analyzed using the analysis of variance (ANOVA) and Tukeys test. In the first experiment, when used deionized water (non-erosive challenges) in intraoral phase, the specimens showed lower values of wear compared with the groups that were eroded with citric acid, whether irradiated or non-irradiated with Er:YAG laser. When erosion with citric acid was performed, Er:YAG laser didnt differ from non-irradiated group. The enamel wear was assessed qualitatively by scanning electron microscopy in 3 specimens of each group. In the second experiment, 48 slabs were divided into two groups according to the surface treatment: irradiated with Er:YAG laser (5,2 J/cm2, 2 Hz, 85 mJ, 10 s) and nonirradiated and subdivided according to the type of wear: erosive (citric acid) and erosive associated with abrasive (citric acid + brushing). The challenges were performed by immersing the devices in 100 mL citric acid and citric acid+brushing on each side of the device. The profilometry and scanning electronic microscopy analysis were performed as described in the procedures of the first experiment. The data from the second study were statistically analyzed separately and independently of the first experiment, using the Kruskal-Wallis test and the Mann-Whitney test.The results of the second experiment showed that after erosive and abrasive challenges, the group irradiated with Er:YAG laser showed lower structure loss than nonirradiated group. The group that suffered erosion and was irradiated, did not differ significantly from the non-irradiated group. Thus, it can be concluded the Er:YAG laser irradiation didnt control the enamel loss when submitted to erosive challenges, however, was able to control the enamel loss when submitted to abrasive challenges. (AU)