Behavior of undifferentiated cells from human umbilical cord vein cultured on nanotextured titanium surface

Author(s)

Gabriela Cerminaro Rodrigues

• Document type: Master's Dissertation
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC)
• Defense date: 2015-10-05

Examining board members

Karina Fittipaldi Bombonato Prado; Ana Paula de Souza Faloni; Selma Siessere

• Advisor: Karina Fittipaldi Bombonato Prado

Abstract

Titanium implants have been extensively used in orthopedics and dentistry, mainly as a replacement for missing teeth. Titanium is a metal implant of choice due to its high biocompatibility and corrosion resistance, as well as absence of immune response. The influence of titanium superficial characteristics on morphology and cell behavior has been broadly investigated, but there are still questions about the interaction between titanium and different types of cells in a microenvironment. The purpose of the present investigation is to characterize the behavior of cells from human umbilical cord vein in contact with a nanotextured titanium surface cultured on osteogenic and non-osteogenic medium. Cells were obtained from umbilical cord subintimal vein layer and cultivated in minimal essential medium. After subconfluence, cells were cultured in 24-well plates (n=5) on pure commercial polished titanium discs (control group) and on nanotextured titanium discs by means of acid etching. The evaluated parameters were cell viability, alkaline phosphatase activity as well as the detection of mineralized nodules. It was also performed quantitative gene expression of genes associated to adhesion and extracellular matrix by means of Real Time PCR. All the assays were performed in triplicate and the data obtained were analyzed by means of statistical test (Sigma Plot) after normality and homogeneity tests. (AU)

• FAPESP's process: 13/19041-9 - Assessment of the behavior of undifferentiated cells human umbilical cord cultured on a surface of nano titanium textured
• Grantee: Gabriela Cerminaro Rodrigues
• Support Opportunities: Scholarships in Brazil - Master