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| Author(s): |
Giovana Anceski Bataglion
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2015-09-25 |
| Examining board members: |
Marcos Nogueira Eberlin;
Luiz Augusto dos Santos Madureira;
Alberto Wisniewski Junior;
Fábio Cesar Gozzo;
Susanne Rath
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| Advisor: | Marcos Nogueira Eberlin |
| Abstract | |
This study employs an integrative approach based on ultrahigh resolution mass spectrometry (UHR-MS), ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (UHPLC¿MS/MS) and comprehensive two-dimensional gas chromatography (GC×GC) for the characterization of sedimentary organic matter. The GC×GC technique was employed for a simultaneous characterization of diverse geochemical biomarkers classes and indicators of petroleum-derived organic matter, eliminating fractionation steps, solvent consumption, and also reducing the analysis time. Bidimensional chromatograms displayed chemical profiles with well-defined regions for isoprenoids, alkylbenzenes, n-alkanes, n-alkanols, n-ketones, n-alkanoic acids, sterols, sterenes, diasterenes, hopanes, triterpenoids, and polycyclic aromatic hydrocarbons. An analytical method based on UHPLC¿MS/MS was developed, validated, and then applied in the determination of sterols and triterpenoids. This method showed acceptable analytical parameters and chromatographic run of ten minutes, eliminating both previous separation and derivatization steps. This approach allowed the evaluation of the organic matter sources and input of domestic sewage in different environments. Direct analysis by UHR-MS with electrospray ionization was employed for the characterization of the polar composition (non-GC amenable material). Diverse classes were identified, representing an expansion of the knowledge regarding sedimentary organic matter composition. The use of the proposed integrative approach based on different chromatographic techniques and mass spectrometry allowed an unprecedented inventory of the chemical composition of sedimentary organic matter, for which the used techniques were complementary (AU) | |
| FAPESP's process: | 12/21395-0 - Molecular characterization of sedimentary organic matter in the Estuary-Lagoon System Cananéia-Iguape by Fourier transform ion cyclotron resonance mass spectrometry |
| Grantee: | Giovana Anceski Bataglion |
| Support Opportunities: | Scholarships in Brazil - Doctorate |