Evaluation of the effect of tooth movement on the development of diabetes-induced neuropathy in rats

Fabiana Furtado Freitas

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Author(s):	Fabiana Furtado Freitas Total Authors: 1
Document type:	Master's Dissertation
Press:	Piracicaba, SP.
Institution:	Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
Defense date:	2014-02-25
Examining board members:	Juliana Trindade Clemente Napimoga; Elizabeth Ferreira Martinez; Everardo Magalhães Carneiro
Advisor:	Juliana Trindade Clemente Napimoga
Abstract
Diabetes is known to result in a greater inflammatory response that in turn accentuated orthodontic tooth movement. Thus, this study aimed to evaluate if the higher inflammatory response induced by the orthodontic tooth movement in diabetic animals changes the neuronal excitability in the trigeminal ganglia. For that, Wistar rats (± 150 g, n=4-6/group) were treated with an intraperitoneal injection of citrate buffer (vehicle; Normoglycemic ¿ NG), streptozotocin 75 mg/kg (Diabetic ¿ DG) or streptozotocin 75 mg/kg + subcutaneous injection of insulin (Diabetic treated with insulin ¿ IG). Twenty-eight days after the treatment, an orthodontic appliance was placed and the tooth movement was evaluated at days 0, 1, 3, 6 and 12. After the corresponding time, the animals were terminally anesthetized and their maxillae, trigeminal ganglia and gingival tissue were removed and submitted to analyze the amount of tooth movement and biochemical analysis (ELISA) to measure the release of glutamate, Tumor Necrose Factor-alpha (TNF-?), Interleukin 1-beta (IL-1?), Substance P (SP) and Calcitonin Gene-Related Peptide (CGRP). The results demonstrated that diabetes accentuated orthodontic tooth movement at days 1, 3 and 6 when compared with NG and IG (p<0.05: Two-way ANOVA, Bonferroni¿s test). Corroborating these results, DG rats demonstrated higher release of TNF-? and IL-1? than that observed for the NG and IG rats (p<0.05). Although the greater inflammatory response induced in DG rats, the release of glutamate, SP and CGRP were significantly reduced (p<0.05). The results suggest that neuronal activation in trigeminal ganglia is reduced in diabetes (AU)

FAPESP's process:	12/02389-0 - Evaluation of the effect of tooth movement on the development of diabetes-induced neuropathy in rats
Grantee:	Fabiana Furtado Freitas
Support Opportunities:	Scholarships in Brazil - Master

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