Use of extracts from leaves and residues of savanna fruit species as natural fungicides in films and edible coatings

Author(s)

Caroline Alves Breda

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos
• Defense date: 2015-08-31

Examining board members

Marta Cristina Teixeira Duarte; Eliane Aparecida Benato Rodrigues da Silva; Izabel Cristina Freitas Moraes; Odilio Benedito Garrido de Assis; Rodney Alexandre Ferreira Rodrigues

• Advisor: Marta Cristina Teixeira Duarte

Abstract

The aim of this work was to evaluate the activity of ethanolic extracts obtained from leaves and residues (peels and seeds) of baru, guavira, macaúba, jatobá and pequi aiming the application as antifungal in films and edible coatings. The extracts were evaluated as to their activity against the phytopathogens Alternaria alternata, A. solani, Botrytis cinerea, Colletotrichum gloeosporioides, Mucor hiemalis, Phytophthora infestans and Venturia pirina, among which only obtained from pequi peel and leaves showed activity against A. alternata, A. solani and V. pirina. Then, the active extracts were evaluated for the effect on the radial growth of the fungus. The results showed an increase in the lag phase duration for A. alternata and A. solani in presence of pequi peel and leaves extracts, while radial growth rate was delayed only for A. solani. Based on results of antifungal activity, the ethanolic extract from pequi peel was selected for chemical fractionation, however, the fractions showed less active than the crude extract. Chemical analysis of this extract, performed by electrospray ionization mass spectrometry revealed the presence of previously known compounds with antifungal activity. Regarding toxicity, pequi peel extract showed relatively low acute oral toxicity and was classified in the category defined by 2000 (AU)

• FAPESP's process: 11/19057-7 - Activity of extracts from leaves and residue of cerrado fruits against fruit phytopathogenic fungi.
• Grantee: Caroline Alves Breda
• Support Opportunities: Scholarships in Brazil - Doctorate