Impact of STIP1 cochaperone depletion on the control of pluripotency, proliferation and differentiation of murine embryonic stem cells.

Author(s)

Jenny Andrea Arévalo Romero

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2017-11-07

Examining board members

Marilene Hohmuth Lopes; Patricia Pereira Coltri; Michele Christine Landemberger; Kil Sun Lee; Chao Yun Irene Yan

• Advisor: Marilene Hohmuth Lopes

Abstract

Stress Inducible Phosphoprotein 1 (STIP1) is a crucial co-chaperonin in mice development. In this context, we studied the functions regulated by STIP1 using murine embryonic stem cells (CTEm). Our study shows a regulatory role for STIP1 in JAK/STAT3 pathway, including the transcription factors NANOG, OCT4 and SOX2, characterizing STIP1 as a regulatory agent in self-renewal and pluripotency in CTEm. In addition, an essential role of STIP1 in differentiation was demonstrated since its expression is required in embryoid bodies (EBs) formation with appropriate size and morphology. Moreover, teratoma formation assays showed inhibited tumor formation and defects in differentiation when formation of mesoderm was favored. Furthermore, were revealed the importance of STIP1 in cell proliferation, since its absence affects the function which was partially rescued after treatment with exogenous STIP1. Thus, our work reveals a central role for STIP1 in CTEm, characterizing new functions to understand the biological role of the co-chaperonin in early mammalian development. (AU)

• FAPESP's process: 14/17385-5 - Impact of the depletion of the co-chaperone STI1 in the control of pluripotency, proliferation and differentiation of murine embryonic stem cells
• Grantee: Jenny Andrea Arévalo Romero
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)