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Chemical analysis of color change and transcriptional profile in flavonoids biosynthesis during flower development of Tibouchina pulchra (Cham.) Cong.

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Author(s):
Fernanda Mendes de Rezende
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB)
Defense date:
Examining board members:
Cláudia Maria Furlan; Igor Cesarino; João Paulo Fabi; Deborah Yara Alves Cursino dos Santos; Patrícia Sartorelli
Advisor: Cláudia Maria Furlan; Maria Magdalena Rossi
Abstract

Floral color change is a widespread phenomenon in angiosperms, but poorly understood from the genetic and chemical point of view. This thesis aimed to investigate this phenomenon in Tibouchina pulcha, a native species whose flowers change from white to dark pink. To reach this goal, flavonoid biosynthetic gene expression and compounds were profiled. By using hyphenated techiniques (UPLC-HRMS and NMR), thirty phenolic compounds were quantified and identified, being sixteen described for the first time in T. pulchra. Moreover, an inedite compound was also identified. Five key genes of the flavonoid biosynthetic pathway were partially cloned, sequenced, and the mRNA levels were analyzed (RT-qPCR) along flower development. Collectively, the obtained results demonstrated that the flower color change in T. pulchra is regulated at transcriptional level. Additionally, a metal quantification suggests that Fe3+ ion might influence the saturation of the color at dark pink stage (AU)

FAPESP's process: 13/10413-0 - Chemical analysis of color change and transcriptional profile in biosynthesis of phenylpropanoids and flavonoids during flower development of Tibouchina pulchra (Cham.) Cogn
Grantee:Fernanda Mendes de Rezende
Support type: Scholarships in Brazil - Doctorate