Epithelial to mesenchymal transition related genes are differentially expressed in clear cell renal cell carcinoma

Author(s)

André Luis Giacometti Conceição

• Document type: Doctoral Thesis
• Press: São José do Rio Preto. 2016-03-09.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto
• Defense date: 2016-02-16
• Advisor: Paula Rahal

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. This carcinoma is histologically characterized by the presence of clear and abundant cytoplasm. The initial process of ccRCC metastatic tumor formation can be attributed to epithelial- mesenchymal transition (EMT). In this study, we sought to identify genes differentially expressed in ccRCC and build a molecular profile of this cancer. We selected genes described in the literature to be related to EMT, cellular differentiation and proliferation. We analyzed the gene and protein expression by quantitative PCR and immunohistochemistry, respectively, and examined possible epigenetic mechanisms that regulate their expression in ccRCC samples and cell lines. OCLN and GAS1 genes were under-expressed in ccRCC, and we report that miR-122 and miR- 34a, respectively, may regulate their expression in this cancer. Furthermore, we showed by quantitative PCR (qPCR) and immunohistochemistry that SLC2A1 was significantly over-expressed in ccRCC. The set of genes identified in this study furthers our understanding of the molecular basis and development of ccRCC. (AU)

• FAPESP's process: 12/08853-0 - Analysis of the expression of genes involved in epithelium mesenchyme transition in renal clear cell carcinoma
• Grantee: Andre Luis Giacometti Conceicao
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)