Study of desication tolerance and longevity in soybean (Glycine max (L.) MERR.) seeds

Author(s)

Bárbara Panoff Valário

• Document type: Doctoral Thesis
• Press: Botucatu. 2016-10-11.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agronômicas. Botucatu
• Defense date: 2016-08-12
• Advisor: Edvaldo Aparecido Amaral da Silva

Abstract

There is no consensus regarding when desiccation tolerance and longevity are acquired in soybean seeds. Therefore, this study aimed at to characterize the acquisition of desiccation tolerance and longevity in soybean seeds and identify proteins associated. The study was performed at the Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Colegue of Agricultual Science-UNESP-Botucatu in collaboration with the Virtual Center for Toxicology (CEVAP), Botucatu-SP and with the Forest Seeds Laboratory at the Federal University of Lavras (UFLA). Seed production was carried out in the crop year 2013/2014 followed by the collection and characterization of seeds at the reproductive stages R5.1, R5.2, R5.3, R5.4, R5.5, R6, R7.1, R7.2, R7.3, R8.1, R8.2, R8.3 and R9. Subsequently, it was performed the determination of water content, dry and fresh weight of the seeds. Then, seeds were dried and stored at 35 °C and 75% relative humidity (RH), to characterize the acquisition of longevity. To know the proteomic profile, proteins were extracted from each stage of development, separated in acrylamide gels and analyzed by ImageMaster Platinum 7.0. The significant spot and unique to each stadium were cut and analyzed by mass spectrometry ESI-QTOF mass. They were sequenced and identified 167 proteins, and thirty-five had the differential expression along the late stage of maturation. The desiccation tolerance was acquired at the stage R7.2 but longevity was acquired at later during seed development. Proteins LEAs MAT1, SBP65 and MP2 are related to desiccation tolerance and those belonging to group 3 (51 kDa, SBP65 and MP2) together with MAT9 and some LEAs group 5 (Small hydrophilic plant seed protein) are related to acquisition of longevity. (AU)

• FAPESP's process: 14/14638-0 - Acquisition of longevity: the proteome study during maturity of soybean (Glycine max (L.) Merr.) seeds
• Grantee: Bárbara Panoff Valário
• Support Opportunities: Scholarships in Brazil - Doctorate