Full text
| |
| Author(s): |
Allison Kleiton dos Anjos
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Rio Claro. 2018-01-15. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro |
| Defense date: | 2017-12-15 |
| Advisor: | Diogo Cavalcanti Cabral de Mello |
| Abstract | |
The hemipterans of the Cicadomorpha infraorder are represented by approximately 30.000 species of sucking insects distributed worldwide. Although they stand out by cause many damages in agriculture and livestock, they are poorly studied regarding the genetic and chromosomal variability of these animals that present holocentric chromosomes. Repetitive DNAs are a useful tools in studies of karyotypic diversification, organization and evolution of genomes. The aim of this work was to contribute with knowledge about the dynamics of the karyotypes of Cicadomorpha and the organization of repetitive DNAs, specifically: I. Analyze the karyotype of species belonging to four families of Cicadomorpha, as well as to characterize the constitutive heterochromatin regarding base pairs richess; II. Infer about the evolutionary dynamics of multigene families through the chromosomal mapping; III. Analyze the chromosomal organization and test the interspecific conservation of the telemetric repeat TTAGGn from the pool of repetitive DNA fraction (C0t fraction) of Mahanarva species and telomeric sequences; IV. Analyze the satellitome of Mahanarva quadripunctata and compare the different satellite DNAs of its genome with that from other species of the genus in order to understand the organization and evolution of satDNAs in this group. The data obtained reveal a wide chromosomal variability among the different families, caused mainly by chromosomal fusions, however within a same family the karyotypes tend to present less variations at the macro-chromosomal level. Although Cicadomorpha exhibit variability in diploid numbers, the organization of repetitive DNAs is highly conserved, even in phylogenetically distant families, suggesting stability. Some data were analyzed based on the phylogeny of the infraorder, suggesting possible ancestral patterns. In addition, the possible causes of variability relative to modal patterns are suggested. The data presented is an advance in the knowledge of the organization of repetitive DNAs in Cicadomorpha, being for some sequences the first time that some study is done. (AU) | |
| FAPESP's process: | 14/06226-3 - Karyotypic structure and evolution among Cercopidae splitebug species (Hemiptera: Auchenorrhyncha) based in the analysis of repetitive DNAs |
| Grantee: | Allison Kleiton do Anjos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |