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| Author(s): |
Darcio Zangirolami Filho
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Jaboticabal. 2018-03-27. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2018-02-05 |
| Advisor: | Carlos Augusto Araujo Valadão |
| Abstract | |
In vitro fertilization and embryo transfer are often used as genetic multipliers in cows of high zootechnical value. Epidural anesthesia is mandatory preceding these procedures, but repeated puncture of the epidural space may cause inflammation and fibrosis, which reduce the effectiveness of the technique in time. The objective of this study was to describe a surgical technique for implantation of an epidural catheter with subcutaneous access portal, and to evaluate the patency this device during eight follicular aspiration cycles in cows. Twelve cows, aged between 3-5 years, weighting 308-518 kg were used in this study. They were allocated in two groups, (A) Nellore and (B) Holstein. Following physical retrain in a hydraulic stock and sedation with 0.04 mg/kg of acepromazine 1% associated with 0.01 mg/Kg of xylazine 2%, administered intramuscularly, local anesthetic block of the sacrococcygeal and right sacral region was made. Then, a 10-cm semicircular followed by skin incision (I-incision), was performed over the sacrococcygeal space, after divulsion of the subcutaneous tissue. Through a Tuohy needle (16G) positioned within the sacrococcygeal joint, ten centimeters of the catheter (17G) were cranially inserted in the epidural space. A second incision (incision-II), four centimeters long, was made at the middle third of the right sacral region. The epidural catheter was transposed from the I-incision to the II-incision through the subcutaneous by using a metallic guide, and promptly connected to the access portal, which was accommodated in the subcutaneous space of the sacral region. Both incisions were sutured with simple interrupted pattern. After 15 days of implantation, the patency of the catheter and portal was confirmed by administration of 3 mL of lidocaine 2%. After 30 days, the local anesthetic was reapplied through the access portal to perform transvaginal follicular aspiration. This procedure was repeated every 30 days for 240 days (8 cycles). Following, 9 months interval a new follicular aspiration was performed on the 510th day following implantation. The surgical technique for the catheter and portal implantation was of medium complexity. Anesthesia was effective for follicular aspiration along the eight cycles. No evidence of portal rejection and total or partial catheter obstruction were noticed. The surgical wounds promptly closed by primary intention. The implantation of the portal and the catheter allowed multiple administrations of local anesthetic optimizing follicular aspiration until the 510th day of implantation. (AU) | |
| FAPESP's process: | 14/11630-8 - Experimental use of Port-a-Cath® implantable epidural access in the analgesia of cows reproductive tract |
| Grantee: | Darcio Zangirolami Filho |
| Support Opportunities: | Scholarships in Brazil - Doctorate |